a band stab pcr.
oh, and this is another solution for my problem:
"Problem: Bright bands in well of agarose gel following electrophoresis [of PCR product].Possible Remedy: Such bands usually result from overamplification of the PCR product or from insufficient dilution of the product prior to electrophoresis. This is also a common result of amplifications from too much genomic DNA. Try diluting the template 100- to 1000-fold."
ps: work, work and work.
*update: eksperimen berjaye! haha...